Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex

Osamu Inoue; Hitomi Taguchi; Toshimitsu Watanabe; Rie Hosoi; Kaoru Kobayashi; Tsunehiko Nishimura; Antony Gee

Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex

Osamu Inoue, Hitomi Taguchi, Toshimitsu Watanabe, Rie Hosoi, Kaoru Kobayashi, Tsunehiko Nishimura, Antony Gee

PET Imaging Centre Facility

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The effects of sodium nitroprusside (SNP) on cerebral blood flow and glucose metabolism were investigated by the microinfusion of SNP into rat cerebral cortex. A significant enhancement in glucose metabolism, as measured using [14C]deoxyglucose (DG), was observed throughout widespread areas of the cerebral cortex within 1 h of microinjection of 50 nmol/microl SNP. Using a kinetic analysis, the increase in glucose metabolism was found to be due to an increase in the phosphorylation of [14C]DG in the brain. On the other hand, regional cerebral blood flow, as measured using [14C]iodoantypirine, was not significantly altered by the SNP infusion. No significant cell death was detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining 1 h after the SNP infusion. The uncoupling of flow and metabolism was almost completely prevented by pretreatment with an NMDA antagonist, MK-801. However, pretreatment with MK-801 did not prevent the SNP-induced neural cell death detected 6 h after the SNP infusion. These results suggest that the SNP-induced uncoupling of flow and metabolism was not directly related to neural cell death in the cerebral cortex.

Original language	English
Pages (from-to)	141-145
Number of pages	5
Journal	Neuroreport
Volume	15
Issue number	1
Publication status	Published - 19 Jan 2004

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title = "Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex",

abstract = "The effects of sodium nitroprusside (SNP) on cerebral blood flow and glucose metabolism were investigated by the microinfusion of SNP into rat cerebral cortex. A significant enhancement in glucose metabolism, as measured using [14C]deoxyglucose (DG), was observed throughout widespread areas of the cerebral cortex within 1 h of microinjection of 50 nmol/microl SNP. Using a kinetic analysis, the increase in glucose metabolism was found to be due to an increase in the phosphorylation of [14C]DG in the brain. On the other hand, regional cerebral blood flow, as measured using [14C]iodoantypirine, was not significantly altered by the SNP infusion. No significant cell death was detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining 1 h after the SNP infusion. The uncoupling of flow and metabolism was almost completely prevented by pretreatment with an NMDA antagonist, MK-801. However, pretreatment with MK-801 did not prevent the SNP-induced neural cell death detected 6 h after the SNP infusion. These results suggest that the SNP-induced uncoupling of flow and metabolism was not directly related to neural cell death in the cerebral cortex.",

author = "Osamu Inoue and Hitomi Taguchi and Toshimitsu Watanabe and Rie Hosoi and Kaoru Kobayashi and Tsunehiko Nishimura and Antony Gee",

year = "2004",

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language = "English",

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TY - JOUR

T1 - Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex

AU - Inoue, Osamu

AU - Taguchi, Hitomi

AU - Watanabe, Toshimitsu

AU - Hosoi, Rie

AU - Kobayashi, Kaoru

AU - Nishimura, Tsunehiko

AU - Gee, Antony

PY - 2004/1/19

Y1 - 2004/1/19

N2 - The effects of sodium nitroprusside (SNP) on cerebral blood flow and glucose metabolism were investigated by the microinfusion of SNP into rat cerebral cortex. A significant enhancement in glucose metabolism, as measured using [14C]deoxyglucose (DG), was observed throughout widespread areas of the cerebral cortex within 1 h of microinjection of 50 nmol/microl SNP. Using a kinetic analysis, the increase in glucose metabolism was found to be due to an increase in the phosphorylation of [14C]DG in the brain. On the other hand, regional cerebral blood flow, as measured using [14C]iodoantypirine, was not significantly altered by the SNP infusion. No significant cell death was detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining 1 h after the SNP infusion. The uncoupling of flow and metabolism was almost completely prevented by pretreatment with an NMDA antagonist, MK-801. However, pretreatment with MK-801 did not prevent the SNP-induced neural cell death detected 6 h after the SNP infusion. These results suggest that the SNP-induced uncoupling of flow and metabolism was not directly related to neural cell death in the cerebral cortex.

AB - The effects of sodium nitroprusside (SNP) on cerebral blood flow and glucose metabolism were investigated by the microinfusion of SNP into rat cerebral cortex. A significant enhancement in glucose metabolism, as measured using [14C]deoxyglucose (DG), was observed throughout widespread areas of the cerebral cortex within 1 h of microinjection of 50 nmol/microl SNP. Using a kinetic analysis, the increase in glucose metabolism was found to be due to an increase in the phosphorylation of [14C]DG in the brain. On the other hand, regional cerebral blood flow, as measured using [14C]iodoantypirine, was not significantly altered by the SNP infusion. No significant cell death was detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining 1 h after the SNP infusion. The uncoupling of flow and metabolism was almost completely prevented by pretreatment with an NMDA antagonist, MK-801. However, pretreatment with MK-801 did not prevent the SNP-induced neural cell death detected 6 h after the SNP infusion. These results suggest that the SNP-induced uncoupling of flow and metabolism was not directly related to neural cell death in the cerebral cortex.

M3 - Article

C2 - 15106846

SN - 0959-4965

VL - 15

SP - 141

EP - 145

JO - Neuroreport

JF - Neuroreport

IS - 1

ER -

Uncoupling of flow and metabolism induced by sodium nitroprusside in rat cerebral cortex

Abstract

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