TY - JOUR
T1 - Understanding Human Epidermal Stem Cells at Single-Cell Resolution
AU - Negri, Victor Augusti
AU - Watt, Fiona M
N1 - Funding Information:
We gratefully acknowledge the advice and practical contributions of members of the wattlab, past and present. This work was supported by grants to FMW from the United Kingdom Medical Research Council ( MR/PO18823/1 ) and the Wellcome Trust ( 206439/Z/17/Z ; WT211276/Z/18/Z ). VAN is the recipient of a National Council for Scientific and Technological Development-Brazil doctoral scholarship. FMW also acknowledges funding from the Department of Health through the National Institute for Health Research comprehensive Biomedical Research Centre award to Guy’s & St Thomas’ NHS Foundation Trust in partnership with King’s College London and King’s College Hospital NHS Foundation Trust.
Funding Information:
We gratefully acknowledge the advice and practical contributions of members of the wattlab, past and present. This work was supported by grants to FMW from the United Kingdom Medical Research Council (MR/PO18823/1) and the Wellcome Trust (206439/Z/17/Z; WT211276/Z/18/Z). VAN is the recipient of a National Council for Scientific and Technological Development-Brazil doctoral scholarship. FMW also acknowledges funding from the Department of Health through the National Institute for Health Research comprehensive Biomedical Research Centre award to Guy's & St Thomas’ NHS Foundation Trust in partnership with King's College London and King's College Hospital NHS Foundation Trust. Conceptualization: FMW; Data Curation: VAN; Formal Analysis: VAN; Funding Acquisition: FMW; Investigation: VAN, FMW; Methodology: VAN, FMW; Project Administration: FMW; Resources: FMW; Software: VAN; Supervision: FMW; Validation: VAN, FMW; Visualization: VAN; Writing - Original Draft Preparation: FMW; Writing - Review & Editing: VAN, FMW
Funding Information:
FMW receives research funding from Unilever and POLA.
Publisher Copyright:
© 2022 The Authors
PY - 2022/8
Y1 - 2022/8
N2 - The human epidermis is one of the first tissues in which the existence of stem cells was recognized and is one of the few in which ex vivo expansion for tissue repair is established clinically. Nevertheless, the nature of stem cells has been elusive. Using clonal growth assays of cultured keratinocytes as a quantitative measure of their abundance, several candidate stem cell markers have been described. Recently, the volume and quality of single-cell RNA-sequencing datasets have increased exponentially, providing new opportunities to explore the nature of epidermal stem cells and test the validity of in vitro experimental models.
AB - The human epidermis is one of the first tissues in which the existence of stem cells was recognized and is one of the few in which ex vivo expansion for tissue repair is established clinically. Nevertheless, the nature of stem cells has been elusive. Using clonal growth assays of cultured keratinocytes as a quantitative measure of their abundance, several candidate stem cell markers have been described. Recently, the volume and quality of single-cell RNA-sequencing datasets have increased exponentially, providing new opportunities to explore the nature of epidermal stem cells and test the validity of in vitro experimental models.
UR - http://www.scopus.com/inward/record.url?scp=85130396236&partnerID=8YFLogxK
U2 - 10.1016/j.jid.2022.04.003
DO - 10.1016/j.jid.2022.04.003
M3 - Review article
C2 - 35570025
SN - 0022-202X
VL - 142
SP - 2061
EP - 2067
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 8
ER -