Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry

O M Kon; B S Sihra; S J Till; C J Corrigan; A B Kay; J A Grant

Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry

O M Kon, B S Sihra, S J Till, C J Corrigan, A B Kay, J A Grant

King's College London

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

IgE-stimulated cultured basophils from atopic subjects are capable of secreting interleukin-4 (IL-4). We describe a flow-cytometric technique which identified intracellular IL-4 in unstimulated basophils unseparated from peripheral blood mononuclear cells (PBMC) in both atopic (AT) and nonatopic (NC) volunteers.

Original language	English
Pages (from-to)	891-6
Number of pages	6
Journal	Allergy
Volume	53
Issue number	9
Publication status	Published - Sept 1998

Keywords

Interferon-gamma
Humans
Asthma
Rhinitis
Antibodies, Monoclonal
Basophils
Allergens
Immunoglobulin E
Adult
Interleukin-4
Flow Cytometry
Middle Aged
Female
Male

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http://www.ncbi.nlm.nih.gov/pubmed/9788692

Cite this

@article{f1fac82a25d944009caf87264f47ac5a,

title = "Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry",

abstract = "IgE-stimulated cultured basophils from atopic subjects are capable of secreting interleukin-4 (IL-4). We describe a flow-cytometric technique which identified intracellular IL-4 in unstimulated basophils unseparated from peripheral blood mononuclear cells (PBMC) in both atopic (AT) and nonatopic (NC) volunteers.",

keywords = "Interferon-gamma, Humans, Asthma, Rhinitis, Antibodies, Monoclonal, Basophils, Allergens, Immunoglobulin E, Adult, Interleukin-4, Flow Cytometry, Middle Aged, Female, Male",

author = "Kon, {O M} and Sihra, {B S} and Till, {S J} and Corrigan, {C J} and Kay, {A B} and Grant, {J A}",

year = "1998",

month = sep,

language = "English",

volume = "53",

pages = "891--6",

journal = "Allergy",

issn = "0105-4538",

publisher = "Blackwell Publishing",

number = "9",

}

TY - JOUR

T1 - Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4

T2 - detection by flow cytometry

AU - Kon, O M

AU - Sihra, B S

AU - Till, S J

AU - Corrigan, C J

AU - Kay, A B

AU - Grant, J A

PY - 1998/9

Y1 - 1998/9

N2 - IgE-stimulated cultured basophils from atopic subjects are capable of secreting interleukin-4 (IL-4). We describe a flow-cytometric technique which identified intracellular IL-4 in unstimulated basophils unseparated from peripheral blood mononuclear cells (PBMC) in both atopic (AT) and nonatopic (NC) volunteers.

AB - IgE-stimulated cultured basophils from atopic subjects are capable of secreting interleukin-4 (IL-4). We describe a flow-cytometric technique which identified intracellular IL-4 in unstimulated basophils unseparated from peripheral blood mononuclear cells (PBMC) in both atopic (AT) and nonatopic (NC) volunteers.

KW - Interferon-gamma

KW - Humans

KW - Asthma

KW - Rhinitis

KW - Antibodies, Monoclonal

KW - Basophils

KW - Allergens

KW - Immunoglobulin E

KW - Adult

KW - Interleukin-4

KW - Flow Cytometry

KW - Middle Aged

KW - Female

KW - Male

M3 - Article

C2 - 9788692

SN - 0105-4538

VL - 53

SP - 891

EP - 896

JO - Allergy

JF - Allergy

IS - 9

ER -

Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry

Abstract

Keywords

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Cite this