Unveiling P. vivax invasion pathways in Duffy-negative individuals

Isabelle Bouyssou; Sara El Hoss; Cécile Doderer-Lang; Matthieu Schoenhals; Lova Tsikiniaina Rasoloharimanana; Inès Vigan-Womas; Arsène Ratsimbasoa; Andargie Abate; Lemu Golassa; Solenne Mabilotte; Pascal Kessler; Micheline Guillotte-Blisnick; Francisco J Martinez; Chetan E Chitnis; John Strouboulis; Didier Ménard

doi:10.1016/j.chom.2023.11.007

Unveiling P. vivax invasion pathways in Duffy-negative individuals

Isabelle Bouyssou, Sara El Hoss, Cécile Doderer-Lang, Matthieu Schoenhals, Lova Tsikiniaina Rasoloharimanana, Inès Vigan-Womas, Arsène Ratsimbasoa, Andargie Abate, Lemu Golassa, Solenne Mabilotte, Pascal Kessler, Micheline Guillotte-Blisnick, Francisco J Martinez, Chetan E Chitnis, John Strouboulis, Didier Ménard

Comprehensive Cancer Centre

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Vivax malaria has long been thought to be absent from sub-Saharan Africa owing to the high proportion of individuals lacking the Duffy antigen receptor for chemokines (DARC) in their erythrocytes. The interaction between P. vivax Duffy-binding protein (PvDBP) and DARC is assumed to be the main pathway used by merozoites to invade reticulocytes. However, the increasing number of reports of vivax malaria cases in genotypically Duffy-negative (DN) individuals has raised questions regarding the P. vivax invasion pathway(s). Here, we show that a subset of DN erythroblasts transiently express DARC during terminal erythroid differentiation and that P. vivax merozoites, irrespective of their origin, can invade DARC+ DN erythroblasts. These findings reveal that a large number of DN individuals may represent a silent reservoir of deep P. vivax infections at the sites of active erythropoiesis with low or no parasitemia, and it may represent an underestimated biological problem with potential clinical consequences in sub-Saharan Africa.

Original language	English
Pages (from-to)	2080-2092.e5
Journal	Cell Host & Microbe
Volume	31
Issue number	12
DOIs	https://doi.org/10.1016/j.chom.2023.11.007
Publication status	Published - 13 Dec 2023

Keywords

Humans
Malaria, Vivax
Antigens, Protozoan
Protozoan Proteins/metabolism
Plasmodium vivax/metabolism
Erythrocytes
Duffy Blood-Group System/genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.chom.2023.11.007

Cite this

Bouyssou, I., El Hoss, S., Doderer-Lang, C., Schoenhals, M., Rasoloharimanana, L. T., Vigan-Womas, I., Ratsimbasoa, A., Abate, A., Golassa, L., Mabilotte, S., Kessler, P., Guillotte-Blisnick, M., Martinez, F. J., Chitnis, C. E., Strouboulis, J., & Ménard, D. (2023). Unveiling P. vivax invasion pathways in Duffy-negative individuals. Cell Host & Microbe, 31(12), 2080-2092.e5. https://doi.org/10.1016/j.chom.2023.11.007

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title = "Unveiling P. vivax invasion pathways in Duffy-negative individuals",

abstract = "Vivax malaria has long been thought to be absent from sub-Saharan Africa owing to the high proportion of individuals lacking the Duffy antigen receptor for chemokines (DARC) in their erythrocytes. The interaction between P. vivax Duffy-binding protein (PvDBP) and DARC is assumed to be the main pathway used by merozoites to invade reticulocytes. However, the increasing number of reports of vivax malaria cases in genotypically Duffy-negative (DN) individuals has raised questions regarding the P. vivax invasion pathway(s). Here, we show that a subset of DN erythroblasts transiently express DARC during terminal erythroid differentiation and that P. vivax merozoites, irrespective of their origin, can invade DARC+ DN erythroblasts. These findings reveal that a large number of DN individuals may represent a silent reservoir of deep P. vivax infections at the sites of active erythropoiesis with low or no parasitemia, and it may represent an underestimated biological problem with potential clinical consequences in sub-Saharan Africa.",

keywords = "Humans, Malaria, Vivax, Antigens, Protozoan, Protozoan Proteins/metabolism, Plasmodium vivax/metabolism, Erythrocytes, Duffy Blood-Group System/genetics",

author = "Isabelle Bouyssou and {El Hoss}, Sara and C{\'e}cile Doderer-Lang and Matthieu Schoenhals and Rasoloharimanana, {Lova Tsikiniaina} and In{\`e}s Vigan-Womas and Ars{\`e}ne Ratsimbasoa and Andargie Abate and Lemu Golassa and Solenne Mabilotte and Pascal Kessler and Micheline Guillotte-Blisnick and Martinez, {Francisco J} and Chitnis, {Chetan E} and John Strouboulis and Didier M{\'e}nard",

year = "2023",

month = dec,

day = "13",

doi = "10.1016/j.chom.2023.11.007",

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Bouyssou, I, El Hoss, S, Doderer-Lang, C, Schoenhals, M, Rasoloharimanana, LT, Vigan-Womas, I, Ratsimbasoa, A, Abate, A, Golassa, L, Mabilotte, S, Kessler, P, Guillotte-Blisnick, M, Martinez, FJ, Chitnis, CE, Strouboulis, J & Ménard, D 2023, 'Unveiling P. vivax invasion pathways in Duffy-negative individuals', Cell Host & Microbe, vol. 31, no. 12, pp. 2080-2092.e5. https://doi.org/10.1016/j.chom.2023.11.007

TY - JOUR

T1 - Unveiling P. vivax invasion pathways in Duffy-negative individuals

AU - Bouyssou, Isabelle

AU - El Hoss, Sara

AU - Doderer-Lang, Cécile

AU - Schoenhals, Matthieu

AU - Rasoloharimanana, Lova Tsikiniaina

AU - Vigan-Womas, Inès

AU - Ratsimbasoa, Arsène

AU - Abate, Andargie

AU - Golassa, Lemu

AU - Mabilotte, Solenne

AU - Kessler, Pascal

AU - Guillotte-Blisnick, Micheline

AU - Martinez, Francisco J

AU - Chitnis, Chetan E

AU - Strouboulis, John

AU - Ménard, Didier

PY - 2023/12/13

Y1 - 2023/12/13

N2 - Vivax malaria has long been thought to be absent from sub-Saharan Africa owing to the high proportion of individuals lacking the Duffy antigen receptor for chemokines (DARC) in their erythrocytes. The interaction between P. vivax Duffy-binding protein (PvDBP) and DARC is assumed to be the main pathway used by merozoites to invade reticulocytes. However, the increasing number of reports of vivax malaria cases in genotypically Duffy-negative (DN) individuals has raised questions regarding the P. vivax invasion pathway(s). Here, we show that a subset of DN erythroblasts transiently express DARC during terminal erythroid differentiation and that P. vivax merozoites, irrespective of their origin, can invade DARC+ DN erythroblasts. These findings reveal that a large number of DN individuals may represent a silent reservoir of deep P. vivax infections at the sites of active erythropoiesis with low or no parasitemia, and it may represent an underestimated biological problem with potential clinical consequences in sub-Saharan Africa.

AB - Vivax malaria has long been thought to be absent from sub-Saharan Africa owing to the high proportion of individuals lacking the Duffy antigen receptor for chemokines (DARC) in their erythrocytes. The interaction between P. vivax Duffy-binding protein (PvDBP) and DARC is assumed to be the main pathway used by merozoites to invade reticulocytes. However, the increasing number of reports of vivax malaria cases in genotypically Duffy-negative (DN) individuals has raised questions regarding the P. vivax invasion pathway(s). Here, we show that a subset of DN erythroblasts transiently express DARC during terminal erythroid differentiation and that P. vivax merozoites, irrespective of their origin, can invade DARC+ DN erythroblasts. These findings reveal that a large number of DN individuals may represent a silent reservoir of deep P. vivax infections at the sites of active erythropoiesis with low or no parasitemia, and it may represent an underestimated biological problem with potential clinical consequences in sub-Saharan Africa.

KW - Humans

KW - Malaria, Vivax

KW - Antigens, Protozoan

KW - Protozoan Proteins/metabolism

KW - Plasmodium vivax/metabolism

KW - Erythrocytes

KW - Duffy Blood-Group System/genetics

U2 - 10.1016/j.chom.2023.11.007

DO - 10.1016/j.chom.2023.11.007

M3 - Article

C2 - 38056460

SN - 1931-3128

VL - 31

SP - 2080-2092.e5

JO - Cell Host & Microbe

JF - Cell Host & Microbe

IS - 12

ER -