Urine protein concentration estimation for biomarker discovery

Hiten Mistry; Kate Bramham; Andrew Weston; Malcolm Ward; Andrew J. Thompson; Lucy Chappell

doi:10.1016/j.preghy.2013.07.001

Urine protein concentration estimation for biomarker discovery

Hiten Mistry, Kate Bramham, Andrew Weston, Malcolm Ward, Andrew J. Thompson, Lucy Chappell

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Original language	English
Journal	Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health
DOIs	https://doi.org/10.1016/j.preghy.2013.07.001
Publication status	Published - 1 Oct 2013

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10.1016/j.preghy.2013.07.001

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title = "Urine protein concentration estimation for biomarker discovery",

abstract = "Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the {\textquoteleft}gold standard{\textquoteright} amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.",

author = "Hiten Mistry and Kate Bramham and Andrew Weston and Malcolm Ward and Thompson, {Andrew J.} and Lucy Chappell",

year = "2013",

month = oct,

day = "1",

doi = "10.1016/j.preghy.2013.07.001",

language = "English",

journal = "Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health",

issn = "2210-7789",

publisher = "Elsevier",

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TY - JOUR

T1 - Urine protein concentration estimation for biomarker discovery

AU - Mistry, Hiten

AU - Bramham, Kate

AU - Weston, Andrew

AU - Ward, Malcolm

AU - Thompson, Andrew J.

AU - Chappell, Lucy

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

AB - Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

U2 - 10.1016/j.preghy.2013.07.001

DO - 10.1016/j.preghy.2013.07.001

M3 - Article

SN - 2210-7789

JO - Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health

JF - Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health

ER -

Urine protein concentration estimation for biomarker discovery

Abstract

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