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Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation: Basophils and Mast Cells

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

W Alelwani , R. A. Alharbi , D Wan, Driton Vllasaliu, F Falcone, S Stolnik

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationBasophils and Mast Cells
PublisherHumana Press
Pages171-180
Number of pages10
Volume2163
ISBN (Electronic)978-1-0716-0696-4
ISBN (Print)978-1-0716-0695-7
DOIs
Published7 Aug 2020

Publication series

NameMethods in Molecular Biology
Volume2163
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

King's Authors

Abstract

Degranulation of mast cells and basophils occurs after the cross-linking of FcεRI receptor-bound IgE by multivalent allergens, resulting in the release of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.

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