Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review
W Alelwani , R. A. Alharbi , D Wan, Driton Vllasaliu, F Falcone, S Stolnik
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Subtitle of host publication | Basophils and Mast Cells |
| Publisher | Humana Press |
| Pages | 171-180 |
| Number of pages | 10 |
| Volume | 2163 |
| ISBN (Electronic) | 978-1-0716-0696-4 |
| ISBN (Print) | 978-1-0716-0695-7 |
| DOIs | |
| Published | 7 Aug 2020 |
| Additional links |
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2163 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Degranulation of mast cells and basophils occurs after the cross-linking of FcεRI receptor-bound IgE by multivalent allergens, resulting in the release of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.
King's College London - Homepage
© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454