@inbook{ae79e8f9f7584a668c78a900b1db6663,
title = "Using the Dunn chemotaxis chamber to analyze primary cell migration in real time",
abstract = "The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, using the Dunn chemotaxis chamber, we describe the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis.",
author = "Sanjay Chaubey and Ridley, {Anne J} and Wells, {Claire M}",
year = "2011",
doi = "10.1007/978-1-61779-207-6_4",
language = "English",
isbn = "978-1-61779-206-9",
volume = "769",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "41--51",
booktitle = "Cell Migration",
}