Using the Dunn chemotaxis chamber to analyze primary cell migration in real time

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Abstract

The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, using the Dunn chemotaxis chamber, we describe the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis.
Original languageEnglish
Title of host publicationCell Migration
Subtitle of host publicationDevelopmental Methods and Protocols
PublisherHumana Press
Pages41-51
Number of pages11
Volume769
ISBN (Electronic)978-1-61779-207-6
ISBN (Print)978-1-61779-206-9
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume769
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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