Validation of an automatic reference region extraction for the quantification of [(18)F]DPA-714 in dynamic brain PET studies

TY - JOUR

T1 - Validation of an automatic reference region extraction for the quantification of [(18)F]DPA-714 in dynamic brain PET studies

AU - García-Lorenzo, Daniel

AU - Lavisse, Sonia

AU - Leroy, Claire

AU - Wimberley, Catriona

AU - Bodini, Benedetta

AU - Remy, Philippe

AU - Veronese, Mattia

AU - Turkheimer, Federico

AU - Stankoff, Bruno

AU - Bottlaender, Michel

PY - 2017/1/1

Y1 - 2017/1/1

N2 - There is a great need for a non-invasive methodology enabling the quantification of translocator protein overexpression in PET clinical imaging. [(18)F]DPA-714 has emerged as a promising translocator protein radiotracer as it is fluorinated, highly specific and returned reliable quantification using arterial input function. Cerebellum gray matter was proposed as reference region for simplified quantification; however, this method cannot be used when inflammation involves cerebellum. Here we adapted and validated a supervised clustering (supervised clustering algorithm (SCA)) for [(18)F]DPA-714 analysis. Fourteen healthy subjects genotyped for translocator protein underwent an [(18)F]DPA-714 PET, including 10 with metabolite-corrected arterial input function and three for a test-retest assessment. Two-tissue compartmental modelling provided [Formula: see text] estimates that were compared to either [Formula: see text] or [Formula: see text] generated by Logan analysis (using supervised clustering algorithm extracted reference region or cerebellum gray matter). The supervised clustering algorithm successfully extracted a pseudo-reference region with similar reliability using classes that were defined using either all subjects, or separated into HAB and MAB subjects. [Formula: see text], [Formula: see text] and [Formula: see text] were highly correlated (ICC of 0.91 ± 0.05) but [Formula: see text] were ∼26% higher and less variable than [Formula: see text]. Reproducibility was good with 5% variability in the test-retest study. The clustering technique for [(18)F]DPA-714 provides a simple, robust and reproducible technique that can be used for all neurological diseases.

AB - There is a great need for a non-invasive methodology enabling the quantification of translocator protein overexpression in PET clinical imaging. [(18)F]DPA-714 has emerged as a promising translocator protein radiotracer as it is fluorinated, highly specific and returned reliable quantification using arterial input function. Cerebellum gray matter was proposed as reference region for simplified quantification; however, this method cannot be used when inflammation involves cerebellum. Here we adapted and validated a supervised clustering (supervised clustering algorithm (SCA)) for [(18)F]DPA-714 analysis. Fourteen healthy subjects genotyped for translocator protein underwent an [(18)F]DPA-714 PET, including 10 with metabolite-corrected arterial input function and three for a test-retest assessment. Two-tissue compartmental modelling provided [Formula: see text] estimates that were compared to either [Formula: see text] or [Formula: see text] generated by Logan analysis (using supervised clustering algorithm extracted reference region or cerebellum gray matter). The supervised clustering algorithm successfully extracted a pseudo-reference region with similar reliability using classes that were defined using either all subjects, or separated into HAB and MAB subjects. [Formula: see text], [Formula: see text] and [Formula: see text] were highly correlated (ICC of 0.91 ± 0.05) but [Formula: see text] were ∼26% higher and less variable than [Formula: see text]. Reproducibility was good with 5% variability in the test-retest study. The clustering technique for [(18)F]DPA-714 provides a simple, robust and reproducible technique that can be used for all neurological diseases.

U2 - 10.1177/0271678X17692599

DO - 10.1177/0271678X17692599

M3 - Article

C2 - 28178885

SN - 0271-678X

SP - 271678X17692599

JO - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

JF - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

ER -