Abstract
A variety of biomaterials have been fabricated to replace tissues, either as an off-the-shelf option or as a cell-seeded construct. The formation of a mature vascular supply throughout such materials is vital to the success of its integration into host tissue, its long-term survival and regeneration of a mature, functional tissue. In this chapter, the development of healthy vasculature is reviewed in the context of tissue engineering. The importance of a healthy blood supply for bone growth and repair is discussed, as well as various pathologies associated with uncontrolled or dysfunctional blood supply, including tumorigenesis. A number of growth factors essential to healthy tissue formation and angiogenesis have been tested and may be used alone, or in combination with biomaterials and either a single cell type or multicellular co-cultures. Stem cell, osteoblast and endothelial cell co-culture techniques were optimised for tissue engineering of vascularised scaffolds, and vessel formation observed.
| Original language | English |
|---|---|
| Title of host publication | Standardisation in Cell and Tissue Engineering |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Woodhead Publishing Limited |
| Chapter | 5 |
| Pages | 77-103, 103a |
| ISBN (Print) | 9780857094193 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- Angiogenesis
- Biomaterials
- Blood supply
- Bone regeneration
- Growth factors
- Tissue engineering
- Vasculogenesis