VEGF profiling and angiogenesis in human microtissues

J M Kelm; C D Sanchez-Bustamante; E Ehler; S P Hoerstrup; V Djonov; L Ittner; M Fussenegger

doi:10.1016/j.jbiotec.2005.03.016

VEGF profiling and angiogenesis in human microtissues

J M Kelm, C D Sanchez-Bustamante, E Ehler, S P Hoerstrup, V Djonov, L Ittner, M Fussenegger

Research output: Contribution to journal › Article › peer-review

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Abstract

Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in microtissues by gravity-enforced self-aggregation in hanging drops we (i) studied size and age-dependent VEGF production of microtissues in comparison to isogenic monolayer cultures, (ii) characterized the self-organization and VEGF-production potential of mixed-cell spheroids, (iii) analyzed VEGF-dependent capillary formation of human umbilical vein endothelial cells (HUVECs) cells coated onto several human primary cell spheroids, and (iv) profiled endostatin action on vascularization in human microtissues. Precise understanding of vascularization in human microtissues may foster advances in clinical tissue implant engineering, tumor treatment, as well as drug discovery and drug-function analysis. (C) 2005 Elsevier B.V. All rights reserved

Original language	English
Pages (from-to)	213 - 229
Number of pages	17
Journal	JOURNAL OF BIOTECHNOLOGY
Volume	118
Issue number	2
DOIs	https://doi.org/10.1016/j.jbiotec.2005.03.016
Publication status	Published - 4 Aug 2005

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10.1016/j.jbiotec.2005.03.016

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title = "VEGF profiling and angiogenesis in human microtissues",

abstract = "Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in microtissues by gravity-enforced self-aggregation in hanging drops we (i) studied size and age-dependent VEGF production of microtissues in comparison to isogenic monolayer cultures, (ii) characterized the self-organization and VEGF-production potential of mixed-cell spheroids, (iii) analyzed VEGF-dependent capillary formation of human umbilical vein endothelial cells (HUVECs) cells coated onto several human primary cell spheroids, and (iv) profiled endostatin action on vascularization in human microtissues. Precise understanding of vascularization in human microtissues may foster advances in clinical tissue implant engineering, tumor treatment, as well as drug discovery and drug-function analysis. (C) 2005 Elsevier B.V. All rights reserved",

author = "Kelm, {J M} and Sanchez-Bustamante, {C D} and E Ehler and Hoerstrup, {S P} and V Djonov and L Ittner and M Fussenegger",

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doi = "10.1016/j.jbiotec.2005.03.016",

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T1 - VEGF profiling and angiogenesis in human microtissues

AU - Kelm, J M

AU - Sanchez-Bustamante, C D

AU - Ehler, E

AU - Hoerstrup, S P

AU - Djonov, V

AU - Ittner, L

AU - Fussenegger, M

PY - 2005/8/4

Y1 - 2005/8/4

N2 - Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in microtissues by gravity-enforced self-aggregation in hanging drops we (i) studied size and age-dependent VEGF production of microtissues in comparison to isogenic monolayer cultures, (ii) characterized the self-organization and VEGF-production potential of mixed-cell spheroids, (iii) analyzed VEGF-dependent capillary formation of human umbilical vein endothelial cells (HUVECs) cells coated onto several human primary cell spheroids, and (iv) profiled endostatin action on vascularization in human microtissues. Precise understanding of vascularization in human microtissues may foster advances in clinical tissue implant engineering, tumor treatment, as well as drug discovery and drug-function analysis. (C) 2005 Elsevier B.V. All rights reserved

AB - Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in microtissues by gravity-enforced self-aggregation in hanging drops we (i) studied size and age-dependent VEGF production of microtissues in comparison to isogenic monolayer cultures, (ii) characterized the self-organization and VEGF-production potential of mixed-cell spheroids, (iii) analyzed VEGF-dependent capillary formation of human umbilical vein endothelial cells (HUVECs) cells coated onto several human primary cell spheroids, and (iv) profiled endostatin action on vascularization in human microtissues. Precise understanding of vascularization in human microtissues may foster advances in clinical tissue implant engineering, tumor treatment, as well as drug discovery and drug-function analysis. (C) 2005 Elsevier B.V. All rights reserved

U2 - 10.1016/j.jbiotec.2005.03.016

DO - 10.1016/j.jbiotec.2005.03.016

M3 - Article

VL - 118

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EP - 229

JO - JOURNAL OF BIOTECHNOLOGY

JF - JOURNAL OF BIOTECHNOLOGY

IS - 2

ER -

VEGF profiling and angiogenesis in human microtissues

Abstract

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