Visible and near infrared autofluorescence and hyperspectral imaging spectroscopy for the investigation of colorectal lesions and detection of exogenous fluorophores

TY - CHAP

T1 - Visible and near infrared autofluorescence and hyperspectral imaging spectroscopy for the investigation of colorectal lesions and detection of exogenous fluorophores

AU - Koh, Kevin R.

AU - Wood, Tobias

AU - Goldin, Robert D.

AU - Yang, Guang-Zhong

AU - Elson, Daniel S.

PY - 2009/2/18

Y1 - 2009/2/18

N2 - Colonoscopy is the most widely prescribed method of colonic imaging for the clinical surveillance of colorectal cancer. This white light-based imaging technique is often used for the detection of adenomatous polyps, the dysplastic precursors of cancer, that are subsequently removed to prevent progression to colonic carcinoma. However, some adenomas are diminutive and flat, making them difficult to discriminate from non-neoplastic tissue even for the most experienced of colonoscopists. We report here an optical system for performing excitation resolved visible and near-infrared (NIR) autofluorescence imaging and hyperspectral reflectance imaging for the investigation of differences in the spectral signatures between neoplastic and non-neoplastic mucosa along the gastrointestinal tract. This is demonstrated on ex vivo colorectal tissues using our multimodal laparoscopic imaging system, which consists of a supercontinuum excitation source and a digital multimirror device (DMD) for tuneable wavelength selection. We also demonstrate the discrimination of exogenous fluorophores applied to the tissue using the Isomap dimensionality reduction technique for visualisation of the acquired image data.

AB - Colonoscopy is the most widely prescribed method of colonic imaging for the clinical surveillance of colorectal cancer. This white light-based imaging technique is often used for the detection of adenomatous polyps, the dysplastic precursors of cancer, that are subsequently removed to prevent progression to colonic carcinoma. However, some adenomas are diminutive and flat, making them difficult to discriminate from non-neoplastic tissue even for the most experienced of colonoscopists. We report here an optical system for performing excitation resolved visible and near-infrared (NIR) autofluorescence imaging and hyperspectral reflectance imaging for the investigation of differences in the spectral signatures between neoplastic and non-neoplastic mucosa along the gastrointestinal tract. This is demonstrated on ex vivo colorectal tissues using our multimodal laparoscopic imaging system, which consists of a supercontinuum excitation source and a digital multimirror device (DMD) for tuneable wavelength selection. We also demonstrate the discrimination of exogenous fluorophores applied to the tissue using the Isomap dimensionality reduction technique for visualisation of the acquired image data.

U2 - 10.1117/12.809313

DO - 10.1117/12.809313

M3 - Conference paper

SN - 9780819474155

VL - 7169

T3 - Progress in Biomedical Optics and Imaging

SP - N/A

BT - Advanced Biomedical and Clinical Diagnostic Systems VII

A2 - MahadevanJansen, A

A2 - VoDinh, T

A2 - Grundfest, WS

PB - SPIE - INT SOC OPTICAL ENGINEERING

CY - Bellingham, Wash.

T2 - Conference on Advanced Biomedical and Clinical Diagnostic Systems VII

Y2 - 25 January 2009 through 26 January 2009

ER -