TY - JOUR
T1 - Waterborne zinc bioaccumulation influences glucose metabolism in orange-spotted grouper embryos
AU - Zeng, Huiling
AU - Zhang, Peifeng
AU - Ye, Hengzhen
AU - Ji, Yuxiang
AU - Hogstrand, Christer
AU - Green, Iain
AU - Xiao, Juan
AU - Fu, Qiongyao
AU - Guo, Zhiqiang
N1 - Funding Information:
The research was financially supported by the National Key Research and Development Program of China ( 2018YFD0900704 ), National Natural Science Foundation of China ( 31872574 , 41866003 , 31960731 ), and Scientific Research Start-up Fund of Hainan University ( KYQD(ZR)1803 ). The authors wish to thank the anonymous reviewers for the valuable comments on the manuscript.
Publisher Copyright:
© 2021 Elsevier Ltd
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/8/15
Y1 - 2021/8/15
N2 - Fish embryos, as an endogenous system, strictly regulate an energy metabolism that is particularly sensitive to environmental pressure. This study used orange-spotted grouper embryos and stable isotope 67Zn to test the hypothesis that waterborne Zn exposure had a significant effect on energy metabolism in embryos. The fish embryos were exposed to a gradient level of waterborne 67Zn, and then sampled to quantify 67Zn bioaccumulation and mRNA expressions of key genes involved glucose metabolism. The results indicated that the bioaccumulated 67Zn generally increased with increasing waterborne 67Zn concentrations, while it tended to be saturated at waterborne 67Zn > 0.7 mg L−1. As we hypothesized, the expression of PK and PFK gene involved glycolysis pathway was significantly up-regulated under waterborne 67Zn exposure >4 mg L−1. Waterborne 67Zn exposure >2 mg L−1 significantly suppressed PCK and G6PC gene expression involved gluconeogenesis pathway, and also inhibited the AKT2, GSK-3beta and GLUT4 genes involved Akt signaling pathway. Our findings first characterized developmental stage-dependent Zn uptake and genotoxicity in fish embryos. We suggest fish embryos, as a small-scale modeling biosystem, have a large potential and wide applicability for determining cytotoxicity/genotoxicity of waterborne metal in aquatic ecosystem.
AB - Fish embryos, as an endogenous system, strictly regulate an energy metabolism that is particularly sensitive to environmental pressure. This study used orange-spotted grouper embryos and stable isotope 67Zn to test the hypothesis that waterborne Zn exposure had a significant effect on energy metabolism in embryos. The fish embryos were exposed to a gradient level of waterborne 67Zn, and then sampled to quantify 67Zn bioaccumulation and mRNA expressions of key genes involved glucose metabolism. The results indicated that the bioaccumulated 67Zn generally increased with increasing waterborne 67Zn concentrations, while it tended to be saturated at waterborne 67Zn > 0.7 mg L−1. As we hypothesized, the expression of PK and PFK gene involved glycolysis pathway was significantly up-regulated under waterborne 67Zn exposure >4 mg L−1. Waterborne 67Zn exposure >2 mg L−1 significantly suppressed PCK and G6PC gene expression involved gluconeogenesis pathway, and also inhibited the AKT2, GSK-3beta and GLUT4 genes involved Akt signaling pathway. Our findings first characterized developmental stage-dependent Zn uptake and genotoxicity in fish embryos. We suggest fish embryos, as a small-scale modeling biosystem, have a large potential and wide applicability for determining cytotoxicity/genotoxicity of waterborne metal in aquatic ecosystem.
KW - Akt signaling pathway
KW - Bioaccumulation
KW - Embryos
KW - Epinephelus coioides
KW - Gluconeogenesis
KW - Glycolysis
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=85106387930&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2021.117325
DO - 10.1016/j.envpol.2021.117325
M3 - Article
C2 - 34030065
AN - SCOPUS:85106387930
SN - 0269-7491
VL - 283
JO - Environmental Pollution
JF - Environmental Pollution
M1 - 117325
ER -
