Wavelength-resolved 3-dimensional fluorescence lifetime imaging

TY - JOUR

T1 - Wavelength-resolved 3-dimensional fluorescence lifetime imaging

AU - Webb, S E D

AU - Leveque-Fort, S

AU - Elson, D S

AU - Siegel, J

AU - Watson, T

AU - Lever, M J

AU - Booth, M

AU - Juskaitis, R

AU - Neil, M A A

AU - Sucharov, L O

AU - Wilson, T

AU - French, P M W

PY - 2002/6

Y1 - 2002/6

N2 - We have developed a compact system for wide-field fluorescence imaging, resolved in three spatial dimensions, lifetime and wavelength, that is based on a gated optical intensifier and an all-solid-state diode pumped Cr:LiSAF oscillator-amplifier system. Exploiting spectral separation, the system has been applied to human teeth, obtaining good lifetime contrast between enamel, dentin and caries. Exploiting spectral separation combined with depth resolution, the study of fluorescent microspheres led to an enhancement in both lifetime contrast and lateral resolution.

AB - We have developed a compact system for wide-field fluorescence imaging, resolved in three spatial dimensions, lifetime and wavelength, that is based on a gated optical intensifier and an all-solid-state diode pumped Cr:LiSAF oscillator-amplifier system. Exploiting spectral separation, the system has been applied to human teeth, obtaining good lifetime contrast between enamel, dentin and caries. Exploiting spectral separation combined with depth resolution, the study of fluorescent microspheres led to an enhancement in both lifetime contrast and lateral resolution.

UR - http://www.scopus.com/inward/record.url?scp=11144358138&partnerID=8YFLogxK

M3 - Conference paper

VL - 12

SP - 279

EP - 283

JO - JOURNAL OF FLUORESCENCE

JF - JOURNAL OF FLUORESCENCE

IS - 2

T2 - 7th Conference on Methods and Applications of Fluorescence (MAF)

Y2 - 1 January 2002

ER -