Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model

Tobias C. Wood*, Camilla Simmons, Samuel A. Hurley, Anthony C. Vernon, Joel Torres, Flavio Dell'Acqua, Steve C R Williams, Diana Cash

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)
288 Downloads (Pure)

Abstract

Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.

Original languageEnglish
Article numbere2632
JournalPeerJ
Volume2016
Issue number11
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • Cuprizone
  • Inflammation
  • Mouse
  • MRI
  • Myelin
  • Quantitative imaging

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