Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X

N Sergent; J A Levitt; M A Green; K Suhling; W Becker

Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X

N Sergent, J A Levitt, M A Green, K Suhling, W Becker (Editor)

Research output: Chapter in Book/Report/Conference proceeding › Conference paper

Abstract

We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals

Original language	English
Title of host publication	Advanced Photon Counting Techniques Ii
Place of Publication	BELLINGHAM
Publisher	SPIE - INT SOC OPTICAL ENGINEERING
Pages	X7710 - X7710
ISBN (Print)	0277-786X
Publication status	Published - 2007
Event	Conference on Advanced Photon Counting Techniques II - Boston, MA Duration: 1 Jan 2007 → …

Publication series

Name	PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)

Conference

Conference	Conference on Advanced Photon Counting Techniques II
City	Boston, MA
Period	1/01/2007 → …

Cite this

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title = "Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X",

abstract = "We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals",

author = "N Sergent and Levitt, {J A} and Green, {M A} and K Suhling and W Becker",

year = "2007",

language = "English",

isbn = "0277-786X",

series = "PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)",

publisher = "SPIE - INT SOC OPTICAL ENGINEERING",

pages = "X7710 -- X7710",

booktitle = "Advanced Photon Counting Techniques Ii",

note = "Conference on Advanced Photon Counting Techniques II ; Conference date: 01-01-2007",

}

Sergent, N, Levitt, JA, Green, MA , Suhling, K & Becker, W (ed.) 2007, Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X. in Advanced Photon Counting Techniques Ii. PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), SPIE - INT SOC OPTICAL ENGINEERING, BELLINGHAM, pp. X7710 - X7710, Conference on Advanced Photon Counting Techniques II, Boston, MA, 1/01/2007.

Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X. / Sergent, N; Levitt, J A; Green, M A et al.
Advanced Photon Counting Techniques Ii. BELLINGHAM: SPIE - INT SOC OPTICAL ENGINEERING, 2007. p. X7710 - X7710 (PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)).

Research output: Chapter in Book/Report/Conference proceeding › Conference paper

TY - CHAP

T1 - Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X

AU - Sergent, N

AU - Levitt, J A

AU - Green, M A

AU - Suhling, K

A2 - Becker, W

PY - 2007

Y1 - 2007

N2 - We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals

AB - We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals

M3 - Conference paper

SN - 0277-786X

T3 - PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)

SP - X7710 - X7710

BT - Advanced Photon Counting Techniques Ii

PB - SPIE - INT SOC OPTICAL ENGINEERING

CY - BELLINGHAM

T2 - Conference on Advanced Photon Counting Techniques II

Y2 - 1 January 2007

ER -

Wide-field photon counting imaging for fluorescence microscopy - art. no. 67710X

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