Wide-field single photon counting imaging with an ultrafast camera and an image intensifier

Gianmarco Zanda; Nicolas Sergent; Mark Green; James A. Levitt; Zdenek Petrasek; Klaus Suhling

doi:10.1016/j.nima.2011.11.087

Wide-field single photon counting imaging with an ultrafast camera and an image intensifier

Gianmarco Zanda^*, Nicolas Sergent, Mark Green, James A. Levitt, Zdenek Petrasek, Klaus Suhling

^*Corresponding author for this work

TU Dresden

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We are reporting a method for wide-field photon counting imaging using a CMOS camera with a 40 kHz frame rate coupled with a three-stage image intensifier mounted on a standard fluorescence microscope. This system combines high frame rates with single photon sensitivity. The output of the phosphor screen, consisting of single-photon events, is collected by a CMOS camera allowing to create a wide-field image with parallel positional and timing information of each photon. Using a pulsed excitation source and a luminescent sample, the arrival time of hundreds of photons can be determined simultaneously in many pixels with microsecond resolution.

Original language	English
Article number	N/A
Pages (from-to)	306-308
Number of pages	3
Journal	Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment
Volume	695
Issue number	N/A
DOIs	https://doi.org/10.1016/j.nima.2011.11.087
Publication status	Published - 11 Dec 2012

Keywords

CMOS
Ultrafast camera
MCP
TCSPC
Image intensifier
FLUORESCENCE LIFETIME
TIME RESOLUTION
MICROSCOPY

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10.1016/j.nima.2011.11.087

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Zanda, G., Sergent, N., Green, M., Levitt, J. A., Petrasek, Z., & Suhling, K. (2012). Wide-field single photon counting imaging with an ultrafast camera and an image intensifier. Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment, 695(N/A), 306-308. Article N/A. https://doi.org/10.1016/j.nima.2011.11.087

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title = "Wide-field single photon counting imaging with an ultrafast camera and an image intensifier",

abstract = "We are reporting a method for wide-field photon counting imaging using a CMOS camera with a 40 kHz frame rate coupled with a three-stage image intensifier mounted on a standard fluorescence microscope. This system combines high frame rates with single photon sensitivity. The output of the phosphor screen, consisting of single-photon events, is collected by a CMOS camera allowing to create a wide-field image with parallel positional and timing information of each photon. Using a pulsed excitation source and a luminescent sample, the arrival time of hundreds of photons can be determined simultaneously in many pixels with microsecond resolution.",

keywords = "CMOS, Ultrafast camera, MCP, TCSPC, Image intensifier, FLUORESCENCE LIFETIME, TIME RESOLUTION, MICROSCOPY",

author = "Gianmarco Zanda and Nicolas Sergent and Mark Green and Levitt, {James A.} and Zdenek Petrasek and Klaus Suhling",

year = "2012",

month = dec,

day = "11",

doi = "10.1016/j.nima.2011.11.087",

language = "English",

volume = "695",

pages = "306--308",

journal = "Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment",

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number = "N/A",

}

Zanda, G , Sergent, N , Green, M , Levitt, JA, Petrasek, Z & Suhling, K 2012, 'Wide-field single photon counting imaging with an ultrafast camera and an image intensifier', Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment, vol. 695, no. N/A, N/A, pp. 306-308. https://doi.org/10.1016/j.nima.2011.11.087

Wide-field single photon counting imaging with an ultrafast camera and an image intensifier. / Zanda, Gianmarco ; Sergent, Nicolas ; Green, Mark et al.
In: Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment, Vol. 695, No. N/A, N/A, 11.12.2012, p. 306-308.

Research output: Contribution to journal › Article › peer-review

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T1 - Wide-field single photon counting imaging with an ultrafast camera and an image intensifier

AU - Zanda, Gianmarco

AU - Sergent, Nicolas

AU - Green, Mark

AU - Levitt, James A.

AU - Petrasek, Zdenek

AU - Suhling, Klaus

PY - 2012/12/11

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N2 - We are reporting a method for wide-field photon counting imaging using a CMOS camera with a 40 kHz frame rate coupled with a three-stage image intensifier mounted on a standard fluorescence microscope. This system combines high frame rates with single photon sensitivity. The output of the phosphor screen, consisting of single-photon events, is collected by a CMOS camera allowing to create a wide-field image with parallel positional and timing information of each photon. Using a pulsed excitation source and a luminescent sample, the arrival time of hundreds of photons can be determined simultaneously in many pixels with microsecond resolution.

AB - We are reporting a method for wide-field photon counting imaging using a CMOS camera with a 40 kHz frame rate coupled with a three-stage image intensifier mounted on a standard fluorescence microscope. This system combines high frame rates with single photon sensitivity. The output of the phosphor screen, consisting of single-photon events, is collected by a CMOS camera allowing to create a wide-field image with parallel positional and timing information of each photon. Using a pulsed excitation source and a luminescent sample, the arrival time of hundreds of photons can be determined simultaneously in many pixels with microsecond resolution.

KW - CMOS

KW - Ultrafast camera

KW - MCP

KW - TCSPC

KW - Image intensifier

KW - FLUORESCENCE LIFETIME

KW - TIME RESOLUTION

KW - MICROSCOPY

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DO - 10.1016/j.nima.2011.11.087

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VL - 695

SP - 306

EP - 308

JO - Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment

JF - Nuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment

IS - N/A

M1 - N/A

ER -

Wide-field single photon counting imaging with an ultrafast camera and an image intensifier

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Keywords

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