Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling; Liisa Maija Hirvonen; Wolfgang Becker; Stefan Smietana; Holger Netz; James Milnes; Thomas Conneely; Alix Marie Le Marois; Ottmar Jagutzki

doi:10.1117/12.2227198

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling, Liisa Maija Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Marie Le Marois, Ottmar Jagutzki

Research output: Contribution to journal › Conference paper › peer-review

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Abstract

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Original language	English
Article number	98580I
Journal	PROCEEDINGS- SPIE THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING
Volume	9858
DOIs	https://doi.org/10.1117/12.2227198
Publication status	Published - 5 May 2016

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2016_Klaus_ProcSPIE_BaltimoreAccepted author manuscript, 457 KBLicence: Other

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title = "Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy",

abstract = "Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy. {\textcopyright} (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).",

author = "Klaus Suhling and Hirvonen, {Liisa Maija} and Wolfgang Becker and Stefan Smietana and Holger Netz and James Milnes and Thomas Conneely and {Le Marois}, {Alix Marie} and Ottmar Jagutzki",

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language = "English",

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TY - JOUR

T1 - Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

AU - Suhling, Klaus

AU - Hirvonen, Liisa Maija

AU - Becker, Wolfgang

AU - Smietana, Stefan

AU - Netz, Holger

AU - Milnes, James

AU - Conneely, Thomas

AU - Le Marois, Alix Marie

AU - Jagutzki, Ottmar

PY - 2016/5/5

Y1 - 2016/5/5

N2 - Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

AB - Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

U2 - 10.1117/12.2227198

DO - 10.1117/12.2227198

M3 - Conference paper

SN - 0277-786X

VL - 9858

JO - PROCEEDINGS- SPIE THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING

JF - PROCEEDINGS- SPIE THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING

M1 - 98580I

ER -

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

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