TY - CHAP
T1 - Wide-field Time-correlated Single Photon Counting-based Fluorescence Lifetime Imaging Microscopy
AU - Suhling, Klaus
AU - Hirvonen, Liisa Maija
AU - Becker, Wolfgang
AU - Smietana, Stefan
AU - Netz, Holger
AU - Milnes, James
AU - Conneely, Thomas
AU - Le Marois, Alix Marie
AU - Jagutzki, Ottmar
AU - Fred, Festy
AU - Petrášek, Zdeněk
AU - Beeby, Andrew
PY - 2019/10/21
Y1 - 2019/10/21
N2 - Wide-field time-correlated single photon counting detection techniques, where the position and the arrival time of the photons are recorded simultaneously using a camera, have made some advances recently. The technology and instrumentation used for this approach is employed in areas such as nuclear science, mass spectroscopy and positron emission tomography, but here, we discuss some of the wide-field TCSPC methods, for applications in fluorescence microscopy. We describe work by us and others as presented in the Ulitima fast imaging and tracking conference at the Argonne National Laboratory in September 2018, from phosphorescence lifetime imaging (PLIM) microscopy on the microsecond time scale to fluorescence lifetime imaging (FLIM) on the nanosecond time scale, and highlight some applications of these techniques.
AB - Wide-field time-correlated single photon counting detection techniques, where the position and the arrival time of the photons are recorded simultaneously using a camera, have made some advances recently. The technology and instrumentation used for this approach is employed in areas such as nuclear science, mass spectroscopy and positron emission tomography, but here, we discuss some of the wide-field TCSPC methods, for applications in fluorescence microscopy. We describe work by us and others as presented in the Ulitima fast imaging and tracking conference at the Argonne National Laboratory in September 2018, from phosphorescence lifetime imaging (PLIM) microscopy on the microsecond time scale to fluorescence lifetime imaging (FLIM) on the nanosecond time scale, and highlight some applications of these techniques.
UR - http://www.scopus.com/inward/record.url?scp=85070733324&partnerID=8YFLogxK
U2 - 10.1016/j.nima.2019.162365
DO - 10.1016/j.nima.2019.162365
M3 - Conference paper
VL - 942
T3 - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
BT - Nuclear Instruments and Methods A
PB - Elsevier
ER -