Wide-field Time-correlated Single Photon Counting-based Fluorescence Lifetime Imaging Microscopy

Klaus Suhling, Liisa Maija Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Marie Le Marois, Ottmar Jagutzki, Festy Fred, Zdeněk Petrášek, Andrew Beeby

Research output: Chapter in Book/Report/Conference proceedingConference paperpeer-review

26 Citations (Scopus)
203 Downloads (Pure)

Abstract

Wide-field time-correlated single photon counting detection techniques, where the position and the arrival time of the photons are recorded simultaneously using a camera, have made some advances recently. The technology and instrumentation used for this approach is employed in areas such as nuclear science, mass spectroscopy and positron emission tomography, but here, we discuss some of the wide-field TCSPC methods, for applications in fluorescence microscopy. We describe work by us and others as presented in the Ulitima fast imaging and tracking conference at the Argonne National Laboratory in September 2018, from phosphorescence lifetime imaging (PLIM) microscopy on the microsecond time scale to fluorescence lifetime imaging (FLIM) on the nanosecond time scale, and highlight some applications of these techniques.
Original languageEnglish
Title of host publicationNuclear Instruments and Methods A
PublisherElsevier
Volume942
DOIs
Publication statusPublished - 21 Oct 2019

Publication series

NameNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
PublisherElsevier
ISSN (Print)0168-9002

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