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Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Research output: Contribution to journalConference paper

Klaus Suhling ; Liisa Maija Hirvonen ; Wolfgang Becker ; Stefan Smietana ; Holger Netz ; James Milnes ; Thomas Conneely ; Alix Marie Le Marois ; Ottmar Jagutzki

Original languageEnglish
Article number98580I
JournalPROCEEDINGS- SPIE THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING
Volume9858
DOIs
StatePublished - 5 May 2016

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Abstract

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

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