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"Young at heart": Regenerative potential linked to immature cardiac phenotypes

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"Young at heart" : Regenerative potential linked to immature cardiac phenotypes. / Gomes, Renata S M; Skroblin, Philipp; Munster, Alex B.; Tomlins, Hannah; Langley, Sarah R.; Zampetaki, Anna; Yin, Xiaoke; Wardle, Fiona C.; Mayr, Manuel.

In: Journal of Molecular and Cellular Cardiology, Vol. 92, 01.03.2016, p. 105-108.

Research output: Contribution to journalArticle

Harvard

Gomes, RSM, Skroblin, P, Munster, AB, Tomlins, H, Langley, SR, Zampetaki, A, Yin, X, Wardle, FC & Mayr, M 2016, '"Young at heart": Regenerative potential linked to immature cardiac phenotypes', Journal of Molecular and Cellular Cardiology, vol. 92, pp. 105-108. https://doi.org/10.1016/j.yjmcc.2016.01.026

APA

Gomes, R. S. M., Skroblin, P., Munster, A. B., Tomlins, H., Langley, S. R., Zampetaki, A., ... Mayr, M. (2016). "Young at heart": Regenerative potential linked to immature cardiac phenotypes. Journal of Molecular and Cellular Cardiology, 92, 105-108. https://doi.org/10.1016/j.yjmcc.2016.01.026

Vancouver

Gomes RSM, Skroblin P, Munster AB, Tomlins H, Langley SR, Zampetaki A et al. "Young at heart": Regenerative potential linked to immature cardiac phenotypes. Journal of Molecular and Cellular Cardiology. 2016 Mar 1;92:105-108. https://doi.org/10.1016/j.yjmcc.2016.01.026

Author

Gomes, Renata S M ; Skroblin, Philipp ; Munster, Alex B. ; Tomlins, Hannah ; Langley, Sarah R. ; Zampetaki, Anna ; Yin, Xiaoke ; Wardle, Fiona C. ; Mayr, Manuel. / "Young at heart" : Regenerative potential linked to immature cardiac phenotypes. In: Journal of Molecular and Cellular Cardiology. 2016 ; Vol. 92. pp. 105-108.

Bibtex Download

@article{967bc1ccb8b44126a40f0054c7b2a594,
title = "{"}Young at heart{"}: Regenerative potential linked to immature cardiac phenotypes",
abstract = "The adult human myocardium is incapable of regeneration; yet, the zebrafish (Danio rerio) can regenerate damaged myocardium. Similar to the zebrafish heart, hearts of neonatal, but not adult mice are capable of myocardial regeneration. We performed a proteomics analysis of adult zebrafish hearts and compared their protein expression profile to hearts from neonatal and adult mice. Using difference in-gel electrophoresis (DIGE), there was little overlap between the proteome from adult mouse (>. 8 weeks old) and adult zebrafish (18 months old) hearts. Similarly, there was a significant degree of mismatch between the protein expression in neonatal and adult mouse hearts. Enrichment analysis of the selected proteins revealed over-expression of DNA synthesis-related proteins in the cardiac proteome of the adult zebrafish heart similar to neonatal and 4 days old mice, whereas in hearts of adult mice there was a mitochondria-related predominance in protein expression. Importantly, we noted pronounced differences in the myofilament composition: the adult zebrafish heart lacks many of the myofilament proteins of differentiated adult cardiomyocytes such as the ventricular isoforms of myosin light chains and nebulette. Instead, troponin I and myozenin 1 were expressed as skeletal isoforms rather than cardiac isoforms. The relative immaturity of the adult zebrafish heart was further supported by cardiac microRNA data. Our assessment of zebrafish and mammalian hearts challenges the assertions on the translational potential of cardiac regeneration in the zebrafish model. The immature myofilament composition of the fish heart may explain why adult mouse and human cardiomyocytes lack this endogenous repair mechanism.",
keywords = "Cardiomyocyte, Myofilament, Proteomics, Regeneration, Stem cell",
author = "Gomes, {Renata S M} and Philipp Skroblin and Munster, {Alex B.} and Hannah Tomlins and Langley, {Sarah R.} and Anna Zampetaki and Xiaoke Yin and Wardle, {Fiona C.} and Manuel Mayr",
year = "2016",
month = "3",
day = "1",
doi = "10.1016/j.yjmcc.2016.01.026",
language = "English",
volume = "92",
pages = "105--108",
journal = "Journal of Molecular and Cellular Cardiology",
issn = "0022-2828",
publisher = "ACADEMIC PRESS INC",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - "Young at heart"

T2 - Regenerative potential linked to immature cardiac phenotypes

AU - Gomes, Renata S M

AU - Skroblin, Philipp

AU - Munster, Alex B.

AU - Tomlins, Hannah

AU - Langley, Sarah R.

AU - Zampetaki, Anna

AU - Yin, Xiaoke

AU - Wardle, Fiona C.

AU - Mayr, Manuel

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The adult human myocardium is incapable of regeneration; yet, the zebrafish (Danio rerio) can regenerate damaged myocardium. Similar to the zebrafish heart, hearts of neonatal, but not adult mice are capable of myocardial regeneration. We performed a proteomics analysis of adult zebrafish hearts and compared their protein expression profile to hearts from neonatal and adult mice. Using difference in-gel electrophoresis (DIGE), there was little overlap between the proteome from adult mouse (>. 8 weeks old) and adult zebrafish (18 months old) hearts. Similarly, there was a significant degree of mismatch between the protein expression in neonatal and adult mouse hearts. Enrichment analysis of the selected proteins revealed over-expression of DNA synthesis-related proteins in the cardiac proteome of the adult zebrafish heart similar to neonatal and 4 days old mice, whereas in hearts of adult mice there was a mitochondria-related predominance in protein expression. Importantly, we noted pronounced differences in the myofilament composition: the adult zebrafish heart lacks many of the myofilament proteins of differentiated adult cardiomyocytes such as the ventricular isoforms of myosin light chains and nebulette. Instead, troponin I and myozenin 1 were expressed as skeletal isoforms rather than cardiac isoforms. The relative immaturity of the adult zebrafish heart was further supported by cardiac microRNA data. Our assessment of zebrafish and mammalian hearts challenges the assertions on the translational potential of cardiac regeneration in the zebrafish model. The immature myofilament composition of the fish heart may explain why adult mouse and human cardiomyocytes lack this endogenous repair mechanism.

AB - The adult human myocardium is incapable of regeneration; yet, the zebrafish (Danio rerio) can regenerate damaged myocardium. Similar to the zebrafish heart, hearts of neonatal, but not adult mice are capable of myocardial regeneration. We performed a proteomics analysis of adult zebrafish hearts and compared their protein expression profile to hearts from neonatal and adult mice. Using difference in-gel electrophoresis (DIGE), there was little overlap between the proteome from adult mouse (>. 8 weeks old) and adult zebrafish (18 months old) hearts. Similarly, there was a significant degree of mismatch between the protein expression in neonatal and adult mouse hearts. Enrichment analysis of the selected proteins revealed over-expression of DNA synthesis-related proteins in the cardiac proteome of the adult zebrafish heart similar to neonatal and 4 days old mice, whereas in hearts of adult mice there was a mitochondria-related predominance in protein expression. Importantly, we noted pronounced differences in the myofilament composition: the adult zebrafish heart lacks many of the myofilament proteins of differentiated adult cardiomyocytes such as the ventricular isoforms of myosin light chains and nebulette. Instead, troponin I and myozenin 1 were expressed as skeletal isoforms rather than cardiac isoforms. The relative immaturity of the adult zebrafish heart was further supported by cardiac microRNA data. Our assessment of zebrafish and mammalian hearts challenges the assertions on the translational potential of cardiac regeneration in the zebrafish model. The immature myofilament composition of the fish heart may explain why adult mouse and human cardiomyocytes lack this endogenous repair mechanism.

KW - Cardiomyocyte

KW - Myofilament

KW - Proteomics

KW - Regeneration

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=84957554924&partnerID=8YFLogxK

U2 - 10.1016/j.yjmcc.2016.01.026

DO - 10.1016/j.yjmcc.2016.01.026

M3 - Article

AN - SCOPUS:84957554924

VL - 92

SP - 105

EP - 108

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

SN - 0022-2828

ER -

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