Zebrafish neural crest: Lessons and tools to study in vivo cell migration

Zain Alhashem; Macarena Alvarez Garcillan Portillo; Mint Ravinand Htun; Anton Gauert; Luis Briones Montecinos; Steffen Härtel; Claudia Linker

doi:10.1007/978-1-0716-0779-4_9

Zebrafish neural crest: Lessons and tools to study in vivo cell migration

Zain Alhashem
, Macarena Alvarez Garcillan Portillo
, Mint Ravinand Htun
, Anton Gauert
, Luis Briones Montecinos
, Steffen Härtel
, Claudia Linker^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Citations (Scopus)

Abstract

The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc
Pages	79-106
Number of pages	28
DOIs	https://doi.org/10.1007/978-1-0716-0779-4_9
Publication status	Published - 1 Jan 2020

Publication series

Name	Methods in Molecular Biology
Volume	2179
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell dissociation
Cell migration
Cell tracking
Clonal analysis
Gal4/Kalt4
In vivo imaging
Photoconversion
Tamoxifen
UAS
Zebrafish

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10.1007/978-1-0716-0779-4_9

Cite this

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abstract = "The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.",

keywords = "Cell dissociation, Cell migration, Cell tracking, Clonal analysis, Gal4/Kalt4, In vivo imaging, Photoconversion, Tamoxifen, UAS, Zebrafish",

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T2 - Lessons and tools to study in vivo cell migration

AU - Alhashem, Zain

AU - Portillo, Macarena Alvarez Garcillan

AU - Htun, Mint Ravinand

AU - Gauert, Anton

AU - Montecinos, Luis Briones

AU - Härtel, Steffen

AU - Linker, Claudia

PY - 2020/1/1

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N2 - The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

AB - The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

KW - Cell dissociation

KW - Cell migration

KW - Cell tracking

KW - Clonal analysis

KW - Gal4/Kalt4

KW - In vivo imaging

KW - Photoconversion

KW - Tamoxifen

KW - UAS

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/85091129021

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DO - 10.1007/978-1-0716-0779-4_9

M3 - Chapter

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AN - SCOPUS:85091129021

T3 - Methods in Molecular Biology

SP - 79

EP - 106

BT - Methods in Molecular Biology

PB - Humana Press Inc

ER -

Zebrafish neural crest: Lessons and tools to study in vivo cell migration

Abstract

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UN SDGs

Keywords

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